Chemiluminescent Substrates (ECL kit)
T-Pro LumiFast Plus Chemiluminescent Substrate Kit
T-Pro LumiLong Plus Chemiluminescent Substrate Kit
T-Pro LumiDura Chemiluminescent Substrate Kit
T-Pro ECL Kit a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The T-Pro ECL Kit offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
T-Pro LumiDura(no.JT96-K006M) (left) VS Millipore (no.WBKLS0500) (right)
The principle of T-Pro LumiFast Plus, LumiLong and Plus & LumiDura Chemiluminescent Substrate Kit is based on chemiluminescent and is very convenient to detect the
Horseradish peroxidase HRP activity in many assays such as Western blotting, Southern and Northern. HRP catalyze the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol by hydrogen peroxide (H2O2).
T-Pro umiFast Plus, LumiLong and Plus & LumiDura Chemiluminescent Substrate Kit can enhance the luminol-dependent chemiluminescent and can be wildly use to detect the present of HRP-conjugated antibodies or streptavidin which binding to antigen or nucleotide sequence respectively.
T-Pro LumiDura Chemiluminescent Substrate Kit detects long-lasting signal. The indicated amount of 293 total cellular lysate is separated by SDS-PAGE electrophoresis. The membrane is blotted with monoclonal primary antibody recognizes actin and then Goat anti-mouse IgG conjugated with HRP. After antibodies blotting, the signal is detected by T-Pro LumiDura Chemiluminescent Substrate, Amersham ECL (GE) & Pierce ECL. The luminescent signal is detected by X-ray film.
T-Pro LumiFast Plus Chemiluminescent Substrate Kit
Signal Duration: 6-8 hours
Detection Method: X-ray film or imaging acquisition system
Suggested Antibody Dilution: Primary: 1/1,000 – 1/2,000, Secondary: 1/10,000 – 1/50,000
Lower Detection Limit: Low-Picogram (10-9) ~ High-Femtogram (10-12)
T-Pro LumiLong Plus Chemiluminescent Substrate Kit
Signal Duration: 12 hours
Detection Method: X-ray film or imaging acquisition system
Suggested Antibody Dilution: Primary: 1/1,000 – 1/5,000, Secondary: 1/20,000 – 1/100,000
Lower Detection Limit: Low-Picogram (10-10) ~ High-Femtogram (10-13)
T-Pro LumiDura Chemiluminescent Substrate Kit
Signal Duration: 12 hours
Detection Method: X-ray film or imaging acquisition system
Suggested Antibody Dilution: Primary: 1/1,000 – 1/10,000, Secondary: 1/50,000 – 1/250,000
Lower Detection Limit: Low-Picogram (10-11) ~ High-Femtogram (10-14)
