Stripping Reagent
T-Pro Western Blot Stripping Reagent
T-Pro Western Blot Stripping Reagent (EX)
• Effective use of samples that are available in limited amounts.
• Comparison of images obtained with different antibodies in the same blot.
• Confirmation of results with the same or different antibodies.
• It is simply more economical and less time consuming to reuse blots for re-probing and mass spectrometry.
Introduction:
Stripping Reagent for Western Blot is formulated to effectively remove the antibodies from Western blot that have been developed with chemiluminescence. The membrane can be nitrocellulose or PVDF/nylon. The stripped membrane can be re-probing for the Western Blot and for mass spectrometry.
Features：
• Strip blot in 5~15 minutes.
• Incubation at room temperature.
• No stench smelling and without addition of 2-mercaptoethanol or its analogs.
• A propriety formulation with an eco-friendly and non-hazardous organic solvent (with light odor) and can completely disassociate the bound antibodies
Protocol of T-Pro Western Blot Stripping Reagent
• Pour 10 ml stripping reagent to a clean container and put the membrane in the container. Make sure that the membrane is fully submerged with the stripping buffer.
• Incubate the membrane in stripping reagent at room temperature for 3 minutes with strong agitation for twice. For the high affinity antibodies, the incubation time need to be optimized, 5~10 minutes stripping at 37℃ is usually sufficient for most of antibodies.
• Wash the membrane twice by using TBS-T or PBS-T at room temperature in large volumes (e.g. 100 ml) for 3 minutes.
T-Pro Stripping Reagent store RT。
