T-Pro Biotechnology

T-Pro NTR I transfection reagent

T-Pro NTR II transfection reagent

T-Pro NTR III transfection reagent

- Low cytotoxicity for most of tumor cell lines and primary cells

- Exceptional transfection efficiency on the experiment for a broad range of cell types

- Efficient transfection with or without serum

- High levels of recombinant protein production

- Simple, robust transfection on procedure

- Efficient transfection for adhesion cells or suspend cells

T-Pro NTR I, NTR II, NTR III transfection is a series of reagents for highly efficient and reproducible transfection of a variety of adherent and non-adherent cells with nucleic acids.

Outstanding non-liposomal transfection reagents 

T-Pro NTR I, NTR II, NTR III provides highly effective and reliable delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells such as human endothelial cells, fibroblasts and hepatocytes. It consists of a proprietary non-liposomal, cationic polymer that is stable, water-soluble and free of any components of animal origin. In contrast to many liposome-based transfection reagents, it shows very low toxicity and is therefore ideally suited even for transfection of very sensitive cells yielding optimal transfection efficiencies and gene expression. Transfection with T-Pro NTR I, NTR II, NTR III typically requires no medium change and it can be used for highly effective delivery of plasmid DNA as well as oligonucleotides and siRNA into adherent and non-adherent cells. It compacts DNA into positively-charged, stable and protective complexes which bind to negatively-loaded cell-surface residues and enter the cell by endocytosis. In the endosome, T-Pro NTR I, NTR II, NTR III acts as a "proton sponge", buffering the endosomal pH that leads to pH inhibition of endosomal nucleases and ensures stability of the complexes. Continuous proton influx leads to osmotic swelling and rupture of the endosome, allowing a very rapid and almost complete release of the nucleic acids into the cytosol. Moreover, T-Pro NTR I, NTR II, NTR III also favors DNA trafficking to the cell nucleus - a property that is particularly useful for transfection of slow-dividing or resting cells.

-   superior transfection efficiencies with many cell types

-   lowest cytotoxicity with most cell lines - gentle to sensitive cells

-   suited for stable and transient transfections

-   broad cell-line spectrum - ideally suited also for primary cell transfection

-   simple, straightforward and time-saving protocol

-   compatible with serum and antibiotics

-   remarkably reproducible results

-   ideal for virus production

-   cell-specific versions for endothelial cells (e.g. HUVEC or HDMEC), macrophages and hepatocytes available

Description:

T-Pro NTR I, II or III Transfection Reagent is a proprietary formulation for the transfection of DNA and RNA into eukaryotic cells and forms the covalent binding with nucleic acid to provide the highly efficiency and low cytotoxicity.

T-Pro NTR I, II or III Transfection Reagent can be used in many cell types including HEK293, 293T, 293E, BHK, CHO, COS1, COS7, HeLa, Vero, PC3, L929, NIH 3T3, Human Foreskin Fibroblasts (HFF) primary cells, Bovine Aorta Endothelial cells (BAEC), Sf9 and Sf21 Furthermore, T-Pro NTR I, II or III Transfection Reagent also has high transfection efficiency when applied in other eukaryotic cells.

Properties

-  Low cytotoxicity for most of tumor cell lines and primary cells

-  Exceptional transfection efficiency of a broad range of cell types

-  Efficient transfection with or without serum

-  High levels of recombinant protein production

-  Simple, robust transfection procedure  

Procedure:

Form the DNA (μg): Complex (for 24 wells plate ) of T-Pro NTR I, II or III Transfection Reagent (μl)

l  30 μl/well in a sterile plastic tube of Add the Serum-free Culture medium (without antibiotics).

l  Add plasmid DNA 1.0 μg/well and mix by gentle pipetting.

l  Add T-Pro NTR I, II or III 1.0~4.0 μl/well and mix by gentle pipetting.

l  Incubate the mixture of DNA and T-Pro NTR I, II or III solution at room temperature for 15 minutes.

l  Add DNA/ T-Pro NTR I, II or III mixture to cells in each well and mix by gentle and shaking plate. Incubate cells in CO₂ incubator for 18~48 hr.

Transfection efficiency of T-Pro NTR