Western Blotting Buffer
Western Blotting Buffer
Sample Buffer
T-Pro Laemmli (SDS sample) Reagent (reducing 4X)
250 mM Tris HCl, 40% Glycerol, 8% Betamercaptoethanol, 0.02% Bromophenol blue and 8% SDS at a pH of 6.8
T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)
250 mM Tris HCl, 40% Glycerol, 0.02% Bromophenol blue and 8% SDS at a pH of 6.8
T-Pro Laemmli (SDS sample) Reagent (reducing 6X)
375 mM Tris HCl, 50% Glycerol, 9% Betamercaptoethanol, 0.03% Bromophenol blue and 9% SDS at a pH of 6.8
T-Pro Laemmli (SDS sample) Reagent (non-reducing 6X)
375 mM Tris HCl, 50% Glycerol, 0.03% Bromophenol blue and 9% SDS at a pH of 6.8
T-Pro LDS Protein sample Reagent (reducing 4X)
988 mM Tris HCl, 2.04 mM EDTA, 40% Glycerol, 8% Betamercaptoethanol, 0.88mM Coomassie blue, 0.7 mM Phenol Red and 8% LDS at a pH of 8.5
T-Pro LDS Protein sample Reagent (non-reducing 4X)
988 mM Tris HCl, 2.04 mM EDTA, 40% Glycerol, 0.88mM Coomassie blue, 0.7 mM Phenol Red and 8% LDS at a pH of 8.5
Gel Casting Buffer
T-Pro Separating or Resolving Buffer (4X)
1.5 M Tris HCl, 0.4% SDS at a pH of 8.8
T-Pro Stacking Buffer (4X)
0.5 M Tris HCl, 0.4% SDS at a pH of 6.8
Running Buffer
T-Pro Tris-Glycine-SDS running buffer (10X)
0.25 M Tris base, 1.92 M Glycine and 1% SDS at a pH8.3
T-Pro Tris-Glycine-Native running buffer (10X)
0.25 M Tris base, 1.92 M Glycine at a pH8.5
T-Pro MOPS/SDS running buffer (20X)
1 M MOPS, 1 M Tris base, 20 mM EDTA and 2% SDS at a pH7.7
Transfer Buffer
T-Pro Transfer Blotting buffer (10X)
0.25 M Tris base, 1.92 M Glycine
Just add methanol according to your protocol.
T-Pro Semi Dry Transfer Blotting buffer (10X)
0.48 M Tris base, 0.39 M Glycine and 0.375% SDS
Just add methanol according to your protocol.
T-Pro HQ Transfer Blotting buffer (20X)
For trans blot transfer
- T-Pro HQ Transfer Blotting buffer (20X) 50 ml with deionized water 850 ml and add of methanol 100ml.
For semi dry transfer
- T-Pro HQ Transfer Blotting buffer (20X) 50 ml with deionized water 400 ml and add of methanol 50ml.
Washing Buffer
T-Pro Phosphate-Buffered Saline (PBS, 10X)
100 mM Phosphate buffer, 27 mM KCl, 1.37 M NaCl at to pH7.4 after 1X dilution
T-Pro Tris-Buffered Saline (TBS, 10X)
0.5 M Tris HCl, 1.5 M NaCl at to pH7.4 after 1X dilution
T-Pro Washing buffer in PBS and Tween-20 (10X)
100 mM Phosphate buffer, 27 mM KCl, 1.37 M NaCl and 1% Tween-20 at to pH7.4 after 1X dilution
T-Pro Washing buffer in TBS and Tween-20 (10X)
0.5 M Tris HCl, 1.5 M NaCl and 1% Tween-20 at to pH7.4 after 1X dilution