T-Pro Biotechnology

T-Pro EZ Gel Solution (6%, 8%, 10%, 12%, 15%)

T-Pro EZ Gel Solution is "ready-to-run" SDS polyacrylamide solutions polymerize into an advanced molecular sieve for the electrophoretic separation of proteins. Because of the advanced buffer chemistry used in the gel matrix solution, T-Pro EZ Gels allow a single separating gel. No stacking gel is required, as the T-Pro EZ Gel Solution proprietary formulation inherently stacks the protein samples during the normal electrophoresis run. Band resolution is unparalleled over a molecular range of 2.5 to 250 kDa. The new hybrid formulation of T-Pro EZ Gel Solution gives these gels an increased gel strength, which allows for easier handling. T-Pro EZ Gel Solution will work with all types of universal electrophoresis apparatus.

The unique acrylamide matrix of T-Pro EZ Gel Solution slows the progression of proteins, eliminating the need for a stacking gel. This not only saves time, but also permits the proteins to run across a longer gel surface, resulting in increased resolution.

T-Pro EZ Gel Solution ensures batch-to-batch consistency for reliable, reproducible results on each gel run. It may be used with all standard SDS-PAGE equipment and all common staining procedures.

T-Pro EZ Gel Solution is compatible with most downstream applications including 1D and 2D gels, Western blotting, protein sequencing and analysis.

T-Pro Gradient Gel Solution

High Resolution SDS-PAGE

The ready-to-pour SDS-PAGE system for high resolution protein gel electrophoresis.

T-Pro Gradient Gel Solution kit is a revolutionary ready-to-pour pre-mixed solution of acrylamide, bis/acrylamide, gel buffer and SDS that enables ultra-fine resolution of protein bands.

T-Pro Gradient Gel Solution kit ensures batch-to-batch consistency for reliable, reproducible results on each gel run. It may be used with all standard SDS-PAGE equipment and all common staining procedures.

T-Pro Gradient Gel Solution kit is compatible with most downstream applications including 1D and 2D gels, Western blotting, protein sequencing.

T-Pro ECL Kit a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The T-Pro ECL Kit offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.

T-Pro BCA Protein Assay Kit

No. JB04-D001 (A 500ML + B 25ML + C 5*1ML/kit)

Reagent A 500ml/bot, Reagent B 25ml/bot, C Albumin Standard (2mg/ml) 5*1ml/vial

Introduction: 

T-Pro BCA Protein Assay Kitis a detergent-compatible formulation based on bicinchoninic acid for the colorimetric detection and quantitation of total protein. This method was base on the reduction of Cu+2 to Cu+1 by protein in an alkaline medium with the highly sensitive and selective colorimetric detection. The purple-colored reaction product of this assay is formed and exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (20-2,000 µg/ml). The reaction occurred in T-Pro BCA Protein Assay Kitis not a true end-point method; that is, the final color continues to develop. However, following incubation, the rate of continued color development is sufficiently slow to allow large numbers of samples to be assayed together. Thus, protein concentrations generally are determined and reported with reference to standards of bovine serum albumin (BSA). A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknown(s) before the concentration of each unknown is determined based on the standard curve.

T-Pro Bradford  Protein Assay kit (1X or 5X)

(1X) No. JB04-D002 (500ML/bot)

(5X) No. JB04-D003 (500ML/bot)

Introduction:

The T-Pro Bradford Protein Assay Kit is a quick and ready-to-use coomassie-binding, colorimetric method for total protein quantitation in an environment containing up to 1% detergent (1% high protein range, 0.1% low protein range). T-Pro Bradford Protein Assay Kit is an improvement over classical Bradford formulations that cannot tolerate detergent contamination of the protein samples. In addition, the T-Pro Bradford Protein Assay Kit reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations. When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to absorbance obtained for a series of standard protein dilutions, which are assayed alongside the samples with unknown protein concentrations