Cell Counting Kit (CCK-8)

 

The T-Pro Cell Counting Kit (CCK-8) is a convenient and robust way of measuring cell viability. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.

Materials Required, Not Supplied

These materials are not included in the kit, but will be required to successfully perform this assay:

 Microplate reader capable of measuring absorbance at OD = 450-460 nm

 96 well plate with clear flat bottom.

The T-Pro Cell Counting Kit (CCK-8) is a convenient and robust way of measuring cell viability. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.

Note:

l  The absorbance of the blank wells may vary depending on the sources of the microtiter plates or the growth media. 

l  Media : Reagent (CCK-8) = 10 : 1